Recurring chromosomal abnormalities have been identified in a variety of cancers, but are most frequently associated with haematological malignancies such as acute myeloid leukemia (AML). These cytogenetic changes often cause gene fusions leading to expression of chimeric oncofusion proteins. In this project you will focus on unravelling the role of these oncofusion proteins in carcinogenesis. Your aim will be to identify the binding regions, the transcriptional consequences and epigenetic features associated with oncofusion protein expression.
You will use next-generation sequencing to identify oncofusion protein target sites, which then will be further characterized, structurally as well as functionally, in relation to the aetiology of acute myeloid leukemia.
Requirement Details:
You should have:
• an MSc degree in Biomedical Sciences/Biology/Molecular Life Sciences/Bioinformatics;
• experience in Molecular and Cell Biology and/or Bioinformatics;
• a strong affinity for fundamental research related to human disease;
• a well-structured working style;
• well-developed social and communication skills.
You are accurate, and motivated to perform cutting-edge research.
Organization Details:
Research at the department of Molecular Biology is aimed at unravelling the molecular basis of development and differentiation emanating from the genome and epigenome in the context of health and disease with an emphasis on cancer.
State-of-the-art technologies are applied ranging from single molecule studies to genome-wide elucidation of genetic and epigenetic pathways and mechanisms.
A next-generation sequencing platform is operational in the department for analysis of transcription factor binding sites, epigenetic marks, DNA methylation and RNA seq at a comprehensive genome-wide scale.
In-depth analysis of genome-wide data is performed and sustained by our own bioinformatic team as well as with outside collaborators.
Moreover, the group participates in a number of international consortia tackling pathways and molecular mechanisms in lineage commitment of (embryonic) stem cells.
Website: http://www.ncmls.nl/molbio/home.htm
Conditions of employment Details:
Employment: 1,0 fte
Maximum salary per month, based on a fulltime employment: € 2,612 gross/month
The starting salary is € 2,042 per month and will increase to € 2,612 per month in the fourth year. PhD scale.
Duration of the contract: 4 years.
Application Deadline:
Application Deadline is 14 January 2011
Further Details:
For further information about this scholarship visit below link.
http://www.ru.nl/newstaff/working_at_radboud/details/details-vacature?recid=501537
Vercelli, Torcy, Nordborg, Blois, Bergen, S. Lanarkshire, Salloniki, Bolzano,Parma, Oulu, Hensies, Amsterdam, Lyon, Hannover, Remscheid, Krakow, Wroclav,
